biotin polyclonal goat anti vascular endothelial growth factor igg solution  (R&D Systems)

Journal: Current Issues in Molecular Biology

Article Title: Effect of Soy Isoflavone on Prostate Cancer Cell Apoptosis Through Inhibition of STAT3, ERK, and AKT

doi: 10.3390/cimb46110743

Figure Lengend Snippet: Effects of genistein on the p53, p21, AKT, ERK, MAPK, STAT3 pathways, and VEGF protein levels in HPrEC and DU145 cells. Cells were treated with increasing concentrations (0–100 μM) of genistein for 48 h. ( A – E ) The cell lysates were subjected to Western blot analysis targeting proteins such as p53, p21, p-AKT, AKT, p-ERK, ERK, p-p38, p38, p-STAT3, STAT3, and VEGF. GEN: genistein; HPrEC: human prostate epithelial cell line.

Article Snippet: Antibodies against p-AKT (cat. no. 9271), AKT (cat. no. 9272), p-ERK (cat. no. 9101), ERK (cat. no. 9102), p-p38 MAPK (cat. no. 9211), p38 MAPK (cat. no. 8690), Bax (cat. no. 5023), B-cell Lymphoma 2 (Bcl-2; cat. no. 2820), poly (ADP-ribose) polymerase (PARP; cat. no. 9542), cleaved PARP (cat. no. 9541), procaspase-3 (cat. no. 9665), and cleaved caspase-3 (cat. no. 9664) were obtained from Cell Signaling Technology, Inc. (Danvers, CO, USA). p53 (cat. no. sc-126), p21 (cat. no. sc-6246), p-STAT3 (cat. no. sc-8059), STAT3 (cat. no. sc-482), vascular endothelial growth factor (VEGF), goat anti-rabbit IgG-HRP (cat. no. sc-2004), and goat anti-mouse IgG-HRP (cat. no. sc-2005) were acquired from Santa-Cruz Biotechnology (Dallas, TX, USA).

Techniques: Western Blot

Journal: Current Issues in Molecular Biology

Article Title: Effect of Soy Isoflavone on Prostate Cancer Cell Apoptosis Through Inhibition of STAT3, ERK, and AKT

doi: 10.3390/cimb46110743

Figure Lengend Snippet: Schematic representation of the proposed mechanism for genistein-induced cytotoxicity in prostate cancer cells. Genistein inhibits the growth of prostate cancer DU145 cells and induces apoptosis by simultaneously inhibiting the activation of STAT3 and other closely related oncogenic kinases, such as AKT, ERK, and p38, and reducing VEGF expression.

Article Snippet: Antibodies against p-AKT (cat. no. 9271), AKT (cat. no. 9272), p-ERK (cat. no. 9101), ERK (cat. no. 9102), p-p38 MAPK (cat. no. 9211), p38 MAPK (cat. no. 8690), Bax (cat. no. 5023), B-cell Lymphoma 2 (Bcl-2; cat. no. 2820), poly (ADP-ribose) polymerase (PARP; cat. no. 9542), cleaved PARP (cat. no. 9541), procaspase-3 (cat. no. 9665), and cleaved caspase-3 (cat. no. 9664) were obtained from Cell Signaling Technology, Inc. (Danvers, CO, USA). p53 (cat. no. sc-126), p21 (cat. no. sc-6246), p-STAT3 (cat. no. sc-8059), STAT3 (cat. no. sc-482), vascular endothelial growth factor (VEGF), goat anti-rabbit IgG-HRP (cat. no. sc-2004), and goat anti-mouse IgG-HRP (cat. no. sc-2005) were acquired from Santa-Cruz Biotechnology (Dallas, TX, USA).

Techniques: Activation Assay, Expressing